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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 1
First page : (46) Last page : (51)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7205

Molecular Identification of Ten Palm Species using DNA Fingerprinting

Ahmad, I.^1*, Khan, S.¹, Naeem, M.¹, Hayat, M.¹, Ahmed, S.², Murtaza, G.¹, Irfan, M.¹

¹University of Agriculture, Faisalabad, Pakistan
²Bahauddin Zakariya University Multan, Pakistan
*Corresponding Author E-mail: plantresearcher@hotmail.com
Received: 7.01.2019 | Revised: 12.02.2019 | Accepted: 20.02.2019  

 ABSTRACT

DNA Fingerprinting is a valuable tool for plant taxonomists. It is an excellent tool for specie identification at molecular level. Several genetic sequences of chloroplast DNA have been proposed as potential fingerprints. But the Scientists have suggested matK and rbcL genes as the universal fingerprints to distinguish most of the plant species. These genetic sequences differ among plant species, but are closely identical in plants of the same species. Following study was conducted for distinguishing various Palm Species through DNA fingerprinting. Ten Palm species were collected from Date Palm Research Station Jhang. DNA was extracted and quantified. Fingerprinting genetic sequences i.e. rbcL and matK were amplified and cloned. The cloned genetic sequences were analyzed for the variation. The sequences were aligned using multiple alignment tool to determine the identity and differences at nucleotide level. It showed 100% similarity of matK and rbcL, amplified from various varieties of P. dactylifera. Variation was observed in the nucleotide sequences of matK and rbcL for seven Palm species.  While two species P. dactylifera and P. sylvestris contain no variation in the nucleotides of the fingerprinting genes, although morphologically they were identified as different species. Although both regions have potential to differentiate most of the Palm species but still more genes are required to be found for species distinction and identification.

Key words: DNA, Fingerprinting, Molecular, Palm, Taxonomy

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Ahmad, I., Khan, S., Naeem, M., Hayat, M., Ahmed, S., Murtaza, G. and Irfan, M., Molecular Identification of Ten Palm Species using DNA Fingerprinting, Int. J. Pure App. Biosci.7(1): 46-51 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7205