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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 1
First page : (247) Last page : (252)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7327

Antibacterial Effects of Green Synthesized AgNPs from Datura metel Leaf Extracts

Jaspreet Kaur¹*, Neha Gupta¹, Manpreet Kaur¹ and Anshu Sibbal Chatli²

¹Department of Biotechnology, ²Department of Microbiology,
Guru Nanak Girls College, Model Town, Ludhiana, Punjab, India
*Corresponding Author E-mail: kjazz042@gmail.com
Received: 12.01.2019 | Revised: 16.02.2019 | Accepted: 13.02.2019  

 ABSTRACT

The silver nanoparticles were synthesized by biological means using Datura metel leaf extracts prepared at different temperature ranges (00C, 600C and 1000C). Synthesis of silver nanoparticles was confirmed by formation of brown color of reaction mixture and characterized using Calorimeter, Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). The diameter of synthesized silver nanoparticles prepared at 600C was found within the range of 18-30 nm. Pseudomonas sp. and Escherichia coli were isolated from soil samples using specific media. Datura metel leaves extracts were used to evaluate their antibacterial effects at two different concentrations (2.5 ml and 4.5 ml) on Pseudomonas sp. and Escherichia Coli. The biggest halo formation was observed at 4.5 ml concentration of the extracts prepared at 600C.

Key words: Antibacterial activity, Datura metel, Silver nanoparticles, SEM, TEM.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kaur, J., Gupta, N., Kaur, M. and Chatli, A. S., Antibacterial Effects of Green Synthesized AgNPs from Datura metel Leaf Extracts, Int. J. Pure App. Biosci.7(1): 247-252 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7327