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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 1
First page : (190) Last page : (198)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7226

Molecular Characterization of Rice (Oryza sativa L.) Genotypes by using DNA Markers

Elizabeth¹, N. M. Shakuntala² and Dewan J. R.³

¹Msc (Agri), Department of Seed Science and Technology, AC Raichur, UAS Raichur
²Professor and Head, Department of Seed Science and Technology, AC Raichur, UAS Raichur
³Assistant Professor, Department of Genetics and Plant Breeding, AC, UAS, Raichur
*Corresponding Author E-mail: elizabethss1212@gmail.com
Received: 16.01.2019  |  Revised: 19.02.2019   |  Accepted: 25.02.2019  

 ABSTRACT

Rice (Oryza sativa L.) is a “Global Grain” cultivated widely across the world and feeds millions of mankind, is the staple food for more than half of the human population, and in Asia alone more than two billion people depend on rice and its products for their food intake. Rice has become deeply embedded in the cultural heritage of Asian societies and is the life, heart and soul of the people throughout Asia and can be considered as “Rice Basket” of the world, as 90 per cent of world’s rice is grown and consumed by 60 per cent of population and where, about two-thirds of world’s poor live (Khush and Virk, 2005). For molecular characterization, ten rice genotypes by using seven SSR primers among these only five primers viz., RM193, RM190, RM186, RM234 and RM223 showed polymorphism in all genotypes, PIC value ranges from 0.36 to 0.39 and major allele frequency ranged from 0.42 (RM 186) to 0.50 (RM 190) with an average of 0.46 per marker. The genetic dissimilarity estimates for ten genotypes were employed to generate dendrogram by using tree construction with NTSYS Software. Cluster I consists of four genotypes (Gangavathi emergency, IET-25546, DRR-Dhan-44 and RP-Bio-226), Cluster II consists of three genotypes (IET-23304, IET-22066 and IABT-17), Cluster III consists of two genotypes (IET-255051 and IET-26282) and Cluster IV consists of one genotype (GNV-11-09). Molecular characterization of the genotypes gives precise information about the extent of genetic diversity which helps in the development of an appropriate breeding programme.

Key words: Rice, Global Grain, Khush and Virk

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Elizabeth, Shakuntala, N. M. and Dewan, J. R., Molecular Characterization of Rice (Oryza sativa L.) Genotypes by using DNA Markers, Int. J. Pure App. Biosci.7(1): 190-198 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7226