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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (330) Last page : (344)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7121

Bitter-Gourd Attenuates Pre-Diabetes Induced Lens Abnormalities in Neonatal Streptizotocin Induced Rat Model

K. Siva Kesavarao¹*, T. Raghavarao² and P. Suryanarayana¹

¹Lipid chemistry Division, National Institute of Nutrition, Hyderabad, Telangana, India         
²Andhra university, Biochemistry Division, Visakhapatnam
*Corresponding Author E-mail: kskraok@gmail.com
Received: 6.11.2018  |  Revised: 10.12.2018   |  Accepted: 16.12.2018  

 ABSTRACT

The main purpose of our study is to evaluate the impact of bitter gourd on long term pre-diabetic induced lens abnormalities caused by neonatal streptozotocin (nSTZ). Male Sprague Dawley (SD) rat pups of two-day old were taken and injected with STZ (90 mg/kg body weight) dissolved in 0.1M citrate buffer, pH 4.5. Control pups  received only vehicle. All rats were maintained on AIN 93G/M diet in individual cages and a subset of pre-diabetic animals received 5% bitter gourd in the diet. Majority of n-STZ injected rats developed impaired glucose tolerance (IGT) by 2 months and maintained PD state even 11 months. The eye of all groups of animals was examined after dilation of pupils using slit lamp microscope during the experimental period and all three group rat eye lens are clear and not developed any lenticular opacfications. A set of lenses was cultured in modified TC199 medium in the presence of 55mM glucose for a period of four days. Interestingly, untreated pre-diabetic rat lens developed early opacification when compared to control animal lens. PD rats shown increased oxidative stress and antioxidant enzymes evidenced by accumulated MDA and SOD in nSTZ-PD rat lens when compared to control rat lens. Bitter guard treated PD rats marginally reduce blood glucose, IGT along with opacification, polyol intermediates (AR, Sorbitol), oxidative stress marker (MDA) and antioxidant enzyme (SOD). In conclusion, nSTZ-PD rat model could aid to investigate IGT-associated lens abnormalities and bitter gourd considerably reduce PD lens abnormalities might be a hypoglycemic or antioxidant property.

Key words: Pre-diabetes, Cataract, Bitter gourd, STZ.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kesavarao, K.S., Raghavarao, T. and Suryanarayana, P., Bitter-Gourd Attenuates Pre-Diabetes Induced Lens Abnormalities in Neonatal Streptizotocin Induced Rat Model, Int. J. Pure App. Biosci.6(6): 330-344 (2018). doi: http://dx.doi.org/10.18782/2320-7051.7121