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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (172) Last page : (179)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7059

In vitro Free Radical Scavenging, Anti-Hyaluronidase and Anti-Elastase Potential of Flaxseed Lignans Concentrate

Jayarama Naik N.¹ and Basavaraj Madhusudhan^{1, 2}

¹Department of Food Technology, Davangere University, Davangere – 577 007, Karnataka, India
²Research Center for Nanoscience and Technology, Department of Biochemistry and Food Technology,
Davangere University, Shivagangothri, Davangere- 577007, Karnataka, India
*Corresponding Author E-mail: prof.basavarajmadhusudhan@gmail.com
Received: 2.11.2018 | Revised: 7.12.2018 | Accepted: 14.12.2018  

 ABSTRACT

The present study was carried out to study the efficiency of flaxseed lignans concentrate (FLC) on hyaluronidase and elastase enzymes inhibition activity. The DPPH and FRAP Assay were carried out using butylated hydroxytoluene and Iron (II) sulfate as standard molecules. The DPPH radical scavenging ability of FLC had the IC50 value of 486 µg ml-1 and FRAP assay of FLC had IC50 value of 74.12 µg ml-1. Furthermore, oleanolic acid was used as reference for enzyme inhibitory assays. The IC50 value for hyaluronidase inhibitory activity of FLC was 10.75 µg ml-1 whereas oleanolic acid has IC50 value of 9.93 µg ml-1. FLC showed elastase inhibition with IC50 value of 51.44 µg ml-1 whereas standard molecule oleanolic acid had IC50 value of 41.72 µg ml-1. It can be summarized from the above observations that flaxseed being a rich source of lignans and rich anti-oxidant properties, the flaxseed lignans concentrate has the potential similar to oleanolic acid and may be utilized as anti-wrinkle agent in cosmetic products.

Key words: Flaxseed, Anti-wrinkle, Anti-aging, Lignans, Enzymes inhibition.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Naik, J.N. and Madhusudhan, B., In vitro Free Radical Scavenging, Anti-Hyaluronidase and Anti-Elastase Potential of Flaxseed Lignans Concentrate, Int. J. Pure App. Biosci.6(6): 172-179 (2018). doi: http://dx.doi.org/10.18782/2320-7051.7059