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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (6) Last page : ( 19)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7081

Molecular and Microscopic Identification of Fungi in Micropropagation of Nodal and Shoot Tip Culture of Orange

Sharmina Yeasmin¹, Md. Samiul Islam1,², Tareq Sujon¹, Razia Sultana¹, Md. Shah Alam¹, Biswanath Sikdar^1,3, Md. Asadul Islam1,³, Md. Khalekuzzaman^1,3*

¹Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi-6205, Bangladesh
²Department of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China
³Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi-6205, Bangladesh
Both the authors contributed equally in the paper and hence will be considered as first author
*Corresponding Author E-mail: kzaman63@gmail.com
Received: 5.11.2018 | Revised: 12.12.2018 | Accepted: 19.12.2018  

 ABSTRACT

The present investigation was designed to throw light on the molecular identification and characterization of specific fungi responsible for the contamination in tissue culture medium of orange to find out cost-effective control measures. Both the fungi isolate 1 and isolate 2 grew optimally at 25⁰C, pH-6, and 15% sugar content. Molecular identifications of those fungi were performed through PCR and a BlastN search of GenBank 18s rDNA revealed that the isolate-1 and isolate-2 had approximately 92% identity with Alternaria alternata (accession no. MH263651) and Fusarium culmorum (accession no. MH261354). In the antimicrobial activity, a plant extract of Ocimum tenuiflorum almost inhibited the growth of isolate-1 and isolate-2 was inhibited by Ficus hispida. The antagonistic test was performed through the post-harvest fungi isolate Alternaria cucumerina (accession no. MH279485) which 18s rDNA was matched 99% identity.1. It showed respectively 33.33% and 47.13% percentage of growth inhibition (PGI) against isolated two fungal strains.

Key words: Micropropagation, Alternaria alternate, Fusarium culmorum, Molecular analysis, Biological control.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Yeasmin, S., Islam, M.S., Sujon, T., Sultana, R., Alam, M.S., Sikdar, B., Islam, M.A., Khalekuzzaman, M., Molecular and Microscopic Identification of Fungi in Micropropagation of Nodal and Shoot Tip Culture of Orange, Int. J. Pure App. Biosci.6(6): 6-19 (2018). doi: http://dx.doi.org/10.18782/2320-7051.7081