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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (1286) Last page : (1291)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7227

Molecular Characterization of Alternaria alternata (Fr.) KeisslerCausing Leaf Blight Disease of Marigold

P. Prathima*, S. Thiruvudainambi and K. Kalpana

Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai-625104, Tamil Nadu, India
*Corresponding Author E-mail: prathimaprakash55@gmail.com
Received: 27.10.2018 | Revised: 30.11.2018 | Accepted: 7.12.2018  

 ABSTRACT

Leaf blight caused by Alternaria sp. is one of the most important and destructive disease of marigold causing severe yield loss in all marigold growing areas of Southern Tamil Nadu. The pathogen Alternaria sp. causing leaf blight disease was isolated from disease infected leaves by tissue segment method and the pure culture was obtained through single hyphal tip method. The isolates proved its pathogenicity on the marigold hybrid tall yellow. Molecular characterization of the isolate at genus level using the ITS primers resulted in amplification of a 528bp fragment and rDNA-ITS region was sequenced. Use of Alternaria species specific primers AaF and AaR confirmed that this isolate as Alternaria alternata at species level. To know the diversity of this isolate, phylogenetic tree analysis was carried out and the results showed that this A. alternata is distinct from the other Alternaria isolate reported earlier from different host plants. In the present study the pathogen causing leaf blight in marigold was isolated and identified as A. alternata through molecular approach at species level.

Key words: Alternaria sp., rDNA-ITS region, Leaf blight, Marigold

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Prathima, P., Thiruvudainambi, S., Kalpana, K., Molecular Characterization of Alternaria alternata (Fr.) KeisslerCausing Leaf Blight Disease of Marigold, Int. J. Pure App. Biosci.6(6): 1286-1291 (2018). doi: http://dx.doi.org/10.18782/2320-7051.7227