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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (1281) Last page : (1285)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7256

Molecular Relationship of Niger Phytoplasma with Pigeonpea and Parthenium Phyllody Phytoplasmas

Mahalingappa Bandakkanavara*, Prameela, H. A., Raghavendra Achari, Manjunath, S. Hurakadli, Kedarnath, Basavaraj, S. and Rangaswamy, K.T.

Department of Plant Pathology, College of Agriculture, UAS, GKVK, Bengaluru-560 065
*Corresponding Author E-mail: mahanteshsb5287@gmail.com
Received: 2.11.2018  |  Revised: 8.12.2018   |  Accepted: 14.12.2018  

 ABSTRACT

Phytoplasmas are prokaryotes that lack a cell wall and are the causal agents of numerous plant diseases. Phytoplasmas inhabit sieve elements in the phloem of plants and are transmitted between plants by phloem-feeding insects. Phytoplasmas are unculturable, phytopathogenic bacteria that cause economic losses worldwide. It is belong to the class of Mollicutes. As unculturable micro-organisms, phytoplasma taxonomy has been based on the use of the 16S rRNA-encoding gene to establish 16SrRNA groups. Pytoplasmas can affect a wide range of plant hosts, including agriculturally and economically important plants, such as agriculture crops, fruit tree, landscape plant and flowers. Since pigeonpea and parthenium generally found in the niger crop ecosystem, also found in naturally infected with phyllody disease. Studies were conducted to know their relationship with the niger phyllody disease. Nested PCR assay of first round PCR product using universal primers R16F2n/R16R2, a PCR product of 1.2 kb specific to the phytoplasmal 16S rDNA regions of pigeonpea and parthenium were obtained, sequenced and compared with niger phyllody 16S rDNA sequence. Upon comparison of phyllody 16S rDNA sequences of niger, parthenium and pigeonpea, it was revealed that, the nucleotide identities among the three phytoplasma ranged from 85-95 per cent. Nucleotide identity of niger with parthenium was 95 per cent and sequence similarity of 85 per cent with pigeonpea. Phylogenetic analysis revealed that niger and parthenium were closely related and clustered together whereas, pigeonpea form a different cluster.

Key word: Phytoplasma, Pigeonpea, Niger, Parthenium and Molecular characterization

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Mahalingappa B., Prameela, H. A.., Achari, R., Manjunath, S. Hurakadli, Kedarnath, Basavaraj, S. and Rangaswamy, K.T., Molecular Relationship of Niger Phytoplasma with Pigeonpea and  Parthenium Phyllody Phytoplasmas, Int. J. Pure App. Biosci.6(6): 1281-1285 (2018). doi: http://dx.doi.org/10.18782/2320-7051.7256