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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 6
First page : (1225) Last page : (1234)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7239

In vitro Antagonistic Activity of Some Actinomycetes and Fungal spp. Isolated from Rhizosphere of Maize against Fusarium sp. by
Bio-control Dual Inoculation Method

Mahadevaprasad G.* and Veerabhadra Swamy A. L.¹*

*Asst. Professor, Dept. of Microbiology, JSS College for Women (Autonomous),
Saraswathipuram, Mysore – 09, Karnataka
*¹Corresponding Author, Asst. Professor, Post Graduate Department of Botany,
JSS College of Arts, Commerce and Science (Autonomous), B.N. Road, Mysore – 25, Karnataka
*Corresponding Author E-mail: gmpjmp77@gmail.com
Received: 11.11.2018 | Revised: 21.12.2018 | Accepted: 28.12.2018  

 ABSTRACT

Rhizosphere actinomycetes are promising biocontrol agents for use in agriculture and have been isolated from various plant species. In the present investigation, actinomycetes were isolated from rhizospheric soil of maize to test antagonistic activity against Fusarium verticillioides and fungal strain (T. viride) were procured from National fungal culture collection of India (NFCCI), T. harzianum and A. niger were isolated from soil to test their antagonistic effects against Fusarium verticillioides. In the present study bio-control dual inoculum method were followed to evaluate the bacterial and fungal strains against the pathogen and reveals that Single Bacterial Inoculum (SBI) of S. cinereus shows more effective in controlling the growth of Fusarium verticillioides (28 mm in treated plate) compare to control (45 mm fungal growth). But in Dual Bacterial Inoculum (DBI), the combination of S. albosporous and S. cinereus shows more effective (08 mm fungal growth in treated plate) compare to control (52 mm fungal growth). Similarly, in Single Fungal Inoculum (SFI), T. harzianum were more effective (18.8 mm fungal growth) and in Dual Fungal Inoculum (DFI), T. viridae and T. harzianum act as a more effective (2.5 mm fungal growth) against Fusarium verticillioides compare to controls (60 mm and 52 mm fungal growth respectively). Overall interpretation of the present work reveals that DBI and DFI were more effective than SBI and SFI against Fusarium verticillioides.

Key words: Antagonistic, Actinomycetes, Phytopathogenic fungi, Rhizosphere and Biocontrol dual inoculation.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Mahadevaprasad, G. and Swamy, V.A.L., In vitro Antagonistic Activity of Some Actinomycetes and Fungal spp. Isolated from Rhizosphere of Maize against Fusarium sp. by Bio-control Dual Inoculation Method, Int. J. Pure App. Biosci. 6(6): 1225-1234 (2018). doi: http://dx.doi.org/10.18782/2320-7051.7239