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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (637) Last page : (648)
Article doi: : http://dx.doi.org/10.18782/2320-6997

Antagonistic Potential of Bioagents against Alternaria brassicicola, the Incitant of Alternaria Leaf Spot of Cabbage

Gunda V. N. S. Madhu Kiran¹*, Thara, S. S.² and Brinda, G. B.³

¹Department of Plant Pathology, ²Assistant Professor, ³Department of Plant Pathology,
College of Agriculture, Vellayani, Kerala Agricultural University, Kerala, India
*Corresponding Author E-mail: madhuabhi154@gmail.com
Received: 4.07.2018 | Revised: 10.08.2018 | Accepted: 16.08.2018  

 ABSTRACT

Cabbage (Brassica oleracea var capitata (L.)) is widely grown cool season vegetable crop all over the world belongs to the family cruciferae. Economic part of cabbage is leaves forming the compact head. Now this cool season vegetable crop is cultivated under roof top conditions all over the Kerala. In recent years, losses has been observed at large scale due to Alternaria leaf spot of cabbage caused by Alternaria brassicicola as the severity of the damage was too high right from seedling stage to harvest stage. Application of chemical fungicides against this disease creates residue problems as economic part is leaf portion. Hence the present study was conducted to provide an effective biological management strategy against this disease. For this, fungi have been isolated from the phyllosphere of healthy cabbage plant in Alternaria disease affected field and the efficacy of fungi was evaluated against Alternaria leaf spot of cabbage by dual culture technique. The results revealed that the fungi Myrothecium inundatum isolated from the phyllosphere of cabbage were found to be an effective bioagent against the leaf spot pathogen Alternaria brassicicola. Along with this the antagonistic potential of two fungal bioagents Trichoderma viride, Trichoderma harzianum and two bacterial bioagents Bacillus subtilis, Pseudomonas fluorescens were evaluated against Alternaria leaf spot of cabbage by dual culture technique. In this method, the highest mycelial growth inhibition was recorded with Trichoderma viride (83.3%) followed by Trichoderma harzianum (80%), Bacillus subtilis (63.3%) and Myrothecium inundatum (55.5%).

Key words: Cabbage, Alternaria leaf spot, Phyllosphere isolation, Molecular identification, Dual culture technique.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Madhu Kiran, G.V.N.S., Thara, S.S. and Brinda, G.B., Antagonistic Potential of Bioagents against Alternaria brassicicola, the Incitant of Alternaria Leaf Spot of Cabbage, Int. J. Pure App. Biosci.6(5): 637-648 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6997