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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (492) Last page : (497)
Article doi: : http://dx.doi.org/10.18782/2320-6986

Field Evaluation of Oil Based Formulations of N. rileyi Against Spodoptera litura in Rabi Groundnut

G. Bindu Bhargavi*, K. Manjula, A. Ramakrishna Rao and B. Ravindra Reddy

Department of Entomology, S.V. Agricultural College, ANGRAU, Tirupati-517 502, A.P
*Corresponding Author E-mail: bindubhargavi134@gmail.com
Received: 26.07.2018 | Revised: 30.08.2018 | Accepted: 7.09.2018   

 ABSTRACT

The liquid formulations of Nomuraea rileyi, an important entomopathogenic fungus were prepared by using two vegetable oils and two mineral oils viz., olive oil, rice bran oil, liquid paraffin oil, heavy grade mineral oil. N. rileyi spore mass was harvested from culture plates and mixed to autoclaved test oils in the proportions of 0.1g (0.5 x 108 spores/0.1 g) and 0.2g (0.1 x 109 spores/0.2 g) per 100ml. Triton-X 100, a wetting agent was also used in two different concentrations i.e., 0.05% and 0.1% for all four test oils. The pathogenicity of N. rileyi conidia was studied at monthly intervals up to 5 months and mortality percentages of third instar larva of  S. litura was calculated.
Among the 16 oil based formulations of N. rileyi tested in the laboratory, based on the better performance, eight were selected for field evaluation against S. litura in Rabi groundnut. In the field also, rice bran oil with 0.2g spores and 0.1ml triton-X 100 oil formulation recorded highest per cent larval reduction of 82 per cent at 20 days after treatment.  Liquid paraffin with 0.2g spores and 0.1ml triton-X 100 and heavy grade mineral oil with 0.2g spores and 0.1ml triton-X 100 oil formulations also stood as next best treatments. Least performance was observed by olive oil with 0.1g spores and 0.05 ml of triton-X 100 oil formulation (49 per cent larval reduction). The remaining formulations recorded 58-71 per cent larval reduction.

Key words: Nomuraea rileyi, Oil formulations, Spodoptera litura, Rabi, Groundnut, Larval reduction.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bhargavi, B.G., Manjula, K., Rao, A.R. and Reddy, B.R., Field Evaluation of Oil Based Formulations of N. rileyi against Spodoptera litura in Rabi Groundnut, Int. J. Pure App. Biosci.6(5): 492-497 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6986