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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 5
First page : (1026) Last page : (1031)
Article doi: : http://dx.doi.org/10.18782/2320-6654

In vitro Screening of Bacterial Endophytes Against Soilborne Fungal Pathogens of Tomato by Volatile Method

Basamma Hadimani* and S. T. Naik

Department of Plant Pathology, University of Agricultural Sciences, Dharwad- 580 005, Karnataka, India
*Corresponding Author E-mail: basamma655@gmail.com
Received: 24.06.2018 | Revised: 11.07.2018 | Accepted: 16.07.2018  

 ABSTRACT

A total of 45 bacterial endophytes were isolated from apparently healthy tomato plant parts viz., root, stem and leaf tissues and evaluated against soil- borne pathogens viz., Sclerotium rolfsii and Rhizoctonia solani by volatile method to test antifungal activity of volatile metabolites of the tomato bacterial endophytes. Among the 22 root endophytic isolates RBDNA-4, RBDLA-5 and RBDDE-14 showed the maximum inhibition against S. rolfsii to the tune of 89.63, 88.52 and 87.41 per cent, respectively. Out of 12 stem endophytic isolates SBDKA-7 and SBDOF-6 showed the maximum inhibition against S. rolfsii to the tune of 89.26 and 88.52 per cent, respectively and isolate SBHKA-2 showed the maximum inhibition against R. solani to the tune of 66.67 per cent followed by SBDOF-6 (54.81%). Out of 11 endophytic leaf isolates, LBHRA-5 showed maximum inhibition against S. rolfsii to the tune of 81.96 per cent followed by LBDHO-2 (23.53 %) and against R. solani only one  isolate LBHRA-5 showed the maximum inhibition to the tune of 55.44 per cent and the remaining all isolates did not show inhibition. Results clearly indicated that bacterial endophytes were more effective in inhibiting the growth of S. rolfsii than the growth of R. solani. Root and stem isolates were more effective than leaf isolates against both pathogens in volatile method.

Key words: Bacterial endophytes, Sclerotium rolfsii, Rhizoctonia solani, Volatile method

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Hadimani, B. and Naik, S. T., In vitro Screening of Bacterial Endophytes Against Soilborne Fungal Pathogens of Tomato by Volatile Method, Int. J. Pure App. Biosci.6(5): 1026-1031 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6654