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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (548) Last page : (555 )
Article doi: : http://dx.doi.org/10.18782/2320-6770

Molecular Identification of High Laccase Producing Enterobacter cloacae strain T137 Based on 16s rRNA Sequencing

Hemaraju S.¹* and Narasegowda P. N.²

¹Department of Biotechnology, Shridevi P.G. Centre Sira Road, Tumkur-572106 India
²Department of Biotechnology, V.V. Puram College of Science, V.V. Puram,
K.R. Road, Banglaore-560004, India
*Corresponding Author E-mail: hemarajubiotech@gmail.com
Received: 16.07.2018 | Revised: 22.08.2018 | Accepted: 27.08.2018  

 

 ABSTRACT

The present study deals with isolation, identification, and analysis of high laccase producing bacteria from forest soil sample through 16S rRNA based molecular technique. Eight laccase producing bacterial isolates were isolated and screened on LB/Cu2+ agar medium containing 10 mM guaiacol for detection of extracellular laccase enzyme. The bacterial isolate (ISL-6) shown high laccase activity in liquid culture (7.12 U/ml) on the 3rd day of incubation at 37°C and pH 7.0 The most potent bacterial isolate-ISL6 was identified as Enterobacter sp. by morphological and biochemical characterization. Further, the Bacterial strain was confirmed through molecular approach. Bacterial 16S rRNA gene was amplified using suitable primers. The amplified 16S rRNA gene sequence was compared with the sequence in the NCBI sequence database. The bacterial strain was identified as Enterobacter cloacae strain T137 (NCBI Gene Bank Accession No: KC764978.1). Phylogenetic and molecular evolutionary analyses were conducted using 16S rRNA sequencing. The sequence when submitted to NCBI gene bank database using BLAST showed 96% maximum identity and E-value equal to 0 for all closely related taxa.

Key words: Enterobacter sp., Laccase, Forest soil, 16S rRNA sequencing, BLAST.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Hemaraju, S. and Narasegowda, P.N., Molecular Identification of High Laccase Producing Enterobacter cloacae strain T137 Based on 16s rRNA Sequencing, Int. J. Pure App. Biosci.6(4): 548-555 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6770