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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (527) Last page : (534)
Article doi: : http://dx.doi.org/10.18782/2320-6377

Indirect Regeneration of Picrorhiza kurroa Royle ex Benth: An Endangered Medicinal Plant for Ex Situ Conservation

Alka Bameta*, Anita Kumari and A. K. Gaur

Laboratory of Bioprospecting and Metabolomics, Molecular Biology and Genetic Engineering, Collage of Basic Sciences and Humanities, GBPUAT, Pantnagar-263145
*Corresponding Author E-mail: alka.bameta@gmail.com
Received: 23.03.2018  |  Revised: 30.04.2018   |  Accepted: 7.05.2018  

 

 ABSTRACT

An efficient rapid plant regeneration protocol has been optimized for Picrorhiza kurroa Royle ex Benth, propagation which is an endangered and venerable medicinal plant. Leaf explants were established in culture vessel containing Murashige and Skoog (MS) basal medium supplemented with different concentrations of phytoregulators namely Indole Butyric Acid (IBA) and Thiodizuron (TDZ) in order to induce callus which was observed at 0.75 mg/l IBA and 0.75mg/l TDZ with an average of 84.33±13 % frequency for callus induction. The highest value for shoot generation frequency 90±8.660254 % was observed with an average shoot number 8.33±1.0 on the medium supplemented with Benzyl Amino Purine (BAP) 0.5mg/l and kinetin 0.75 mg/l. These micro shoots were sub cultured on the root generation medium for complete regeneration of plantlets on MS medium supplemented with concentrations of 2,4-Dichlorophenoxyacetic acid (2, 4-D) ranging from 0.0mg/l - 1.0 mg/l and Naphthalene Acetic Acid (NAA) ranging from 0.0mg/l - 1.0 mg/l. Maximum root generation frequency was recorded at 2, 4-D (0.5mg/l) and NAA (0.4 mg/l) with an average percentage of 86.33±9.53 with an average number of roots 8.0±0.0.

Key words: Indirect regeneration, Medicinal plants, Picrorhiza kurroa Royle ex Benth.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bameta, A., Kumari, A. and Gaur, A. K., Indirect Regeneration of Picrorhiza kurroa Royle ex Benth: An Endangered Medicinal Plant for Ex Situ Conservation, Int. J. Pure App. Biosci.6(4): 527-534 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6377