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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (456) Last page : (461 )
Article doi: : http://dx.doi.org/10.18782/2320-6269

In Vitro Regeneration of Papaya (Carica papaya L.) Variety Surya

Ramesh, A. N.¹, Santhosh, D. B.¹*, Suresh, D.², Radha Krishna.², Rashmi, K.², and Hajira Khanm.²

¹Department of Plant Biotechnology, University of Agriculture sciences, Bengaluru
²Department of Plant Biotechnology, University of Agriculture and Horticultural sciences, Shivamogga
*Corresponding Author E-mail: santhuroyal99@gmail.com
Received: 20.02.2018  |  Revised: 27.03.2018   |  Accepted: 5.04.2018  

 

 ABSTRACT

An efficient protocol was developed for micro-propagation of Indian papaya (Carica papaya L.) var. Surya. Shoot bud induction occurred on culturing immature zygotic embryos segments in MS (Murashige andSkoog, 1962) medium, followed by transfer onto shoot multiplication, elongation and rooting media. ATreatments with various growth regulators resulted in the production of callus initially followed by induction of shoot buds on subculture to the same medium. among the various growth regulators used viz., BAP, Kinetin, TDZ or 2, 4-D, Maximum per cent of (91.7) callus induction was observed with TDZ at 0.1 mg/l. GA3 at al concentrations was found to induce elongation of shoot buds and maximum number of elongated shoots/ explants (14) was obtained at 1.0 mg/l and thereafter there was a decline in the number of elongated shoots/ explant. The same concentration was found to give rise to maximum extent of elongation with shoots measuring 3.0 cm. Rooting was observed only at 2.0 and 3.0 mg/l with 14.2 % and 28.4% rooting response. The in vitro-raised plantlets were successfully hardened first under culture room conditions, then in greenhouse with 70 % survival rate.

Key words:   Carica papaya, Immature zygotic embryos , Thidiazuron

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Ramesh, A.N., Santhosh, D.B., Suresh, D., Radha Krishna., Rashmi, K., and Hajira Khanm., In Vitro Regeneration of Papaya (Carica papaya L.) Variety Surya, Int. J. Pure App. Biosci.6(4): 456-461 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6269