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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (394) Last page : (400)
Article doi: : http://dx.doi.org/10.18782/2320-6570

Comparative Genetic Variation Among Alternaria brassicae Isolates Infecting Oilseed Brassica In India

Sheetal Chanyal¹, Snigdha Srivastava², Prabhu Dayal Meena³ and Gohar Taj⁴*

¹Junior Research Fellow, ²Ph.D Scholar, ⁴Associate Professor,
^1,2,4Department of Molecular Biology & Genetic Engineering, College of Basic Sciences & Humanities, G.B. Pant University of Agriculture & Technology, District - Udham Singh Nagar, Pantnagar, Uttarakhand,  India
³Principal Scientist, ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur-321303 Rajasthan, India
*Corresponding Author E-mail: gohartajkhan@rediffmail.com
Received: 4.06.2018 | Revised: 11.07.2018   | Accepted: 20.07.2018  

 

 ABSTRACT

Alternaria blight caused by Alternaria brassicae is among the most devastating disease of brassica crop. The present study was carried out to know about the cultural and morphological variability among the different Alternaria brassicae isolates collected from geographically diverse regions of India. Rapid amplified polymorphic DNA (RAPD) markers was used to know  the genetic relationships among the different isolates .Sixteen 10-mer primers of arbitrary nucleotide sequences were tested for amplification of Alternaria brassicae DNA by using PCR. Of these nine primers amplified the fungal genomic DNA from all the twenty isolates and produced reproducible RAPD profiles. The UPGMA analysis showed that the isolates collected from different regions can be classified into four major group. A close relationship was revealed between two isolates based on the cluster analysis data. Our results proved the existence of genetic variability among Alternaria brassicae isolates infecting oilseed brassica which is not based on their location and could be utilized for development of resistance against this pathogen.

Key words: Alternaria brassicae, PCR, RAPD markers.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Chanyal, S., Srivastava, S., Meena, P.D. and Taj, G., Comparative Genetic Variation among Alternaria Brassicae Isolates Infecting Oilseed Brassica in India, Int. J. Pure App. Biosci.6(4): 394-400 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6570