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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (353) Last page : (362 )
Article doi: : http://dx.doi.org/10.18782/2320-6855

Screening and 16S rDNA Identification of PSB (Phosphate Solubilizing Bacteria)   

Aditi Tripathi¹* and Farhat Banu²

*¹Research Scholar, Faculty of Science,
Pacific Academy of Higher Education and Research University, Udaipur- 313024;
²Department of Botany, Govt. Meera Girls College, Udaipur, Rajasthan, India
*Corresponding Author E-mail: adibiotech.bn@gmail.com
Received: 1.09.2017  |  Revised: 28.09.2017   |  Accepted: 8.10.2017  

 

 ABSTRACT

The present study was conducted to analyze microbial diversity of Coriandrum sativum rhizosphere and to isolate phosphate solubilizing bacteria from soils of Hadoti, Rajasthan, India. In total thirty bacteria were isolated from different soil samples collected from Digod, Dingli, Modak, Mandola, Bundi, Munderi, Alnawar and Antah area of Hadoti region. These soil samples have been analyzed for their Organic carbon, available nitrogen, electrical conductance (EC), pH, and temperature. Maximum EC 0.22 dS/m was recorded in Baran district and minimum EC 0.07 dS/m was recorded in Jhalawar district, while maximum pH was recorded 8.4 in Kota district of Rajasthan. Based on their size of halo zone formation on pikovaskaya medium, total ten bacterial isolates were selected as efficient phosphate solubilizing bacterial isolates. The Maximum phosphate solubilization in medium was observed in bacterial isolates COR-61. These isolates were also screened for IAA production, citrate utilization, catalase production, and to utilize different sources of carbohydrates and production of the specific enzymes. On the basis of morphological and biochemical characterization of the isolates we have identified them as members of the following species: Pseudomonas putida, Bacillus altitudinis, Micrococcus luteus, Bacillus subterraneus and Planococcus refietoensis. All five phosphate solubilizing bacteria isolated from rhizospheric soil of coriander could efficiently solubilize tricalcium phosphate in the medium which could possibly help for future application in sustainable production of agriculture crops.

Key words: Coriander seed spice crop, Rhizosphere, Plant growth promoting rhizobacteria (PGPR), Phosphate solubilizing bacteria, biochemical characterization and 16S rDNA molecular analysis.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Tripathi, A and Banu, F., Screening and 16S rDNA Identification of PSB (Phosphate Solubilizing Bacteria), Int. J. Pure App. Biosci.6(4): 353-362 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6855