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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (276) Last page : (290)
Article doi: : http://dx.doi.org/10.18782/2320-5946

In vitro Plant Regeneration in Gymnema sylvestre R. Br.:  Influence of Season and Subculture Induced Oxidative Stress
 

Veerashree V.¹ and Vadlapudi Kumar^1,2*

¹Department of Biochemistry, Kuvempu University-P.G. Centre, Shivagangothri, Davangere, Karnataka, India
²Department of Biochemistry, Davangere University, Shivagangothri, Davangere – 577 002, Karnataka, India
*Corresponding Author E-mail: veeke2012@gmail.com
Received: 27.10.2017  |  Revised: 24.11.2017   |  Accepted: 3.12.2017  

 

 ABSTRACT

Nodal segments from field grown, Gymnema sylvestre plants were cultured on Murashige and Skoog’s (MS) medium supplemented with benzyl adenine (BA), Kinetin and naphthalene acetic acid (NAA) during different seasons. Explanting season between November-March was found to be ideal to obtain optimal culture initiation and regeneration with less contamination. Shoot proliferation was highest on MS basal medium supplemented with 2 mgL-1 BA, 0.2 mgL-1 kinetin and 0.05 mgL-1 NAA. Best rooting response was obtained on half-strength MS medium supplemented with IBA (3 mgL-1). Hardening of the rooted plants was successful on soil: sand: vermicomposte (2:1:1) potting mixture. Active proliferation and growth of explants and plantlets, and also subculture induced oxidative stress were confirmed determining the changes in antioxidants and antioxidant molecules.  The concentrations of different antioxidants and activities of antioxidant enzymes varied with the stage of morphogenesis and subculture. Steady increase in ascorbate (ASC) content was noted throughout regeneration, while higher levels of reduced glutathione (GSH) content was observed during shoot proliferation and rooting. Increase in the activities of ascorbate peroxidase (APX), catalase (CAT) and guaiacol peroxidase (POX) was noticed during shoot organogenesis, while peak activity of GR was noticed during rooting. Increased levels of SOD and GST activities were observed after second subculture, which might contribute in circumventing stress and their involvement in stress tolerance. 

Key words: Gymnema sylvestre; Seasonal influence; Oxidative stress; Antioxidants; Antioxidant enzymes.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Veerashree V. and Kumar, V., In vitro Plant Regeneration in Gymnema sylvestre R. Br.:  Influence of Season and Subculture Induced Oxidative Stress, Int. J. Pure App. Biosci.6(4): 276-290 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5946