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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (134) Last page : (140)
Article doi: : http://dx.doi.org/10.18782/2320-6616

In vitro Callus Induction in Guggul [Commiphora wightii (Arnott)]-
An Endangered Medicinal Plant

Kumawat, S.*, Kumar, R., Shekhawat, K., Kumawat, K., Choudhary, R., Verma R.¹ and Jakhar, M. L.²

¹Research Scholar and 2Professor
Department of Plant Breeding and Genetics, SKN Agriculture University, Jobner
*Corresponding Author E-mail: kumawatswarnlata.sk@gmail.com
Received: 15.06.2018  |  Revised: 22.07.2018   |  Accepted: 29.07.2018  

 

 ABSTRACT

Leaf explant and nodal segment explants of guggul were placed on Murashige and Skoog Medium, supplemented with different concentration of cytokinins and auxins alone or in combination for callus induction. In leaf explants maximum callus induction  was observed on a medium containing 2.0 mg/l 2, 4-D with 100 per cent frequency followed by 5.0 mg/l Kn+0.5 mg/l NAA with 100 per cent frequency. Whereas, maximum callus induction from nodal segment explant was observed on a medium containing 5.0 mg/l BAP+5.0 mg/l NAA with 100 per cent frequency.

Key words: Guggul, Callus induction, Tissue culture, In vitro.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumawat, S., Kumar, R., Shekhawat, K., Kumawat, K., Choudhary, R., Verma, R. and Jakhar, M.L., In vitro Callus Induction in Guggul [Commiphora wightii (Arnott)]- An Endangered  Medicinal Plant , Int. J. Pure App. Biosci.6(4): 134-140 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6616