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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (756) Last page : (761)
Article doi: : http://dx.doi.org/10.18782/2320-6947

Efficacy of Oil Based Formulations of Nomuraea rileyi (Farlow) Samson against Pupae of Spodoptera litura In vitro

G. Bindu Bhargavi*, K. Manjula, A. Ramakrishna Rao and B. Ravindra Reddy

Department of Entomology, S.V. Agricultural College, ANGRAU, Tirupati-517 502, A.P.
*Corresponding Author E-mail: bindubhargavi134@gmail.com
Received: 20.06.2018 | Revised: 24.07.2018   | Accepted: 1.08.2018  

 

 ABSTRACT

The liquid formulations of Nomuraea rileyi, an important entomopathogenic fungus were prepared by using two vegetable oils and two mineral oils viz., olive oil, rice bran oil, liquid paraffin oil, heavy grade mineral oil. N. rileyi spore mass was harvested from culture plates and mixed to autoclaved test oils in the proportions of 0.1g (0.5 x 108 spores/0.1 g) and 0.2g (0.1 x 109 spores/0.2 g) per 100ml. Triton-X 100, a wetting agent was also used in two different concentrations i.e., 0.05% and 0.1% for all four test oils. The pathogenicity of N. rileyi conidia was studied at monthly intervals up to 5 months and mortality percentages of pupae of S. litura was calculated.
Among the 16 oil based formulations of N. rileyi, rice bran oil with 0.2g spores and 0.1ml triton-X 100 oil formulation recorded highest pupal mortality of 60-74 per cent followed by liquid paraffin with 0.2g spores and 0.1ml triton-X 100 and heavy grade mineral oil with 0.2g spores and 0.1ml triton-X 100 oil formulation which recorded 56-70 and 55-69 per cent respectively. The remaining formulations recorded 19-68 per cent pupal mortality.

Key words: Nomuraea rileyi, Oil formulations, Vegetable oils, Mineral oils, Triton-X 100, Pupal mortality.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bhargavi, G.B., Manjula, K., Rao, A.R. and Reddy, B.R., Efficacy of Oil Based Formulations of Nomuraea rileyi (Farlow) Samson against Pupae of Spodoptera litura In vitro, Int. J. Pure App. Biosci.6(4): 756-761 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6947