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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (751) Last page : (755)
Article doi: : http://dx.doi.org/10.18782/2320-6537

Viability of Nomuraea rileyi Conidia in Vegetable and Mineral Oil Based Formulations

G. Bindu Bhargavi*, K. Manjula, A. Ramakrishna Rao and B. Ravindra Reddy

Department of Entomology, S.V. Agricultural College, ANGRAU, Tirupati-517 502, A.P.
*Corresponding Author E-mail: bindubhargavi134@gmail.com
Received: 29.05.2018 | Revised: 27.06.2018   | Accepted: 4.07.2018  

 

 ABSTRACT

The liquid formulations of Nomuraea rileyi, an important entomopathogenic fungus were prepared by using two vegetable oils and two mineral oils viz., olive oil, rice bran oil, liquid paraffin oil, heavy grade mineral oil. N. rileyi spore mass was harvested from culture plates and mixed to autoclaved test oils in the proportions of 0.1g (0.5 x 108 spores/0.1 g) and 0.2g (0.1 x 109 spores/0.2 g) per 100ml. Triton-X 100, a wetting agent was also used in two different concentrations i.e., 0.05% and 0.1% for all four test oils. The viability of N. rileyi conidia was studied at monthly intervals up to 5 months and germination percentages of N. rileyi conidia was calculated. Among the 16 oil based formulations of N. rileyi, rice bran oil with 0.2g spores and 0.1ml triton-X 100 oil formulation recorded highest conidial germination. It was ranged from 68-93 per cent up to 150 days. Liquid paraffin with 0.2g spores and 0.1ml triton-X 100 and heavy grade mineral oil with 0.2g spores and 0.1ml triton-X 100 oil formulations recorded 64-90 per cent and 62-89 per cent conidial germination respectively. The relatively lowest conidial germination of 35-71 per cent was observed in olive oil with 0.1g spores and 0.05 ml of triton-X 100 oil formulation. The remaining formulations recorded 35-87 per cent conidial germination.

Key words:Nomuraea rileyi, Oil formulations, Vegetable oils, Mineral oils, Triton-X 100, Viability.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bhargavi, G.B., Manjula, K., Ramakrishna Rao, A. and Ravindra Reddy, B., Viability of Nomuraea rileyi Conidia in Vegetable and Mineral Oil Based Formulations, Int. J. Pure App. Biosci.6(4): 751-755 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6537