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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 3
First page : (637) Last page : (642)
Article doi: : http://dx.doi.org/10.18782/2320-6198

Evaluation of n-Alkane Contents in Spirulina platensis under UV-B Stress by GC-MS

Radha Gupta*

Plant Molecular Biology and Biotechnology, College of Agriculture, RVSKVV, Gwalior
*Corresponding Author E-mail: radhagi80@rediffmail.com
Received: 25.01.2018  |  Revised: 28.02.2018   |  Accepted: 7.03.2018  

 

 ABSTRACT

n-Alkanes variability in response to UV-B stress has been investigated in the cyanobacterium Spirulina platensis. Saturated hydrocarbons of UV-B treated and untreated cells of S. platensis were separated and identified by gas chromatography-mass spectrometry (GC-MS) using serially coupled capillary column and obtained light chain n-alkanes (C9–C20) 78% and heavy chain n-alkanes (C21–C34) 22% in the UV-B treated cells of S. platensis. In contrast, UV-B–untreated cells of S. platensis had dominance of only long chain 71% and 29% light chain n-alkanes.49% of short chain n-alkanes were increased in UV-B treated cells of S. platensis compared to very low level in its UV-B untreated counterpart. Our finding suggest that in S. platensis under UV-B stress the short chain of n-alkane and the level of tetradecane (C14H30) and heptadecane (C17H36) were  also increased  compared to UV-B untreated counterpart for protection of the cell  in maintaining the vital cellular functions in S.  paltensis under UV-B stress.

Key words:  Spirulina, UV-B stress, n-Alkanes and GC-MS

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Gupta, R., Evaluation of n-Alkane Contents in Spirulina platensis under UV-B Stress by GC-MS, Int. J. Pure App. Biosci.6(3): 637-642 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6198