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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 3
First page : (44) Last page : (48)
Article doi: : http://dx.doi.org/10.18782/2320-7051.6589

An Efficient Inoculation Method for Artificial Screening of Sheath Blight in Rice

Suryawanshi Padmaja Pralhad¹* and P. U. Krishnaraj^2
1Department of Biotechnology, University of Agricultural Sciences, Dharwad - 580005, Karnataka, India
²Department of Agricultural Microbiology, University of Agricultural Sciences, Dharwad - Karnataka, India
*Corresponding Author E-mail: padmajaps87@gmail.com
Received: 10.05.2018 | Revised: 13.06.2018   | Accepted: 20.06.2018  

 ABSTRACT

Sheath blight (Rhizoctonia solani) of rice is an economically important pathogen of rice worldwide. The unavailability of a robust screening method in the greenhouse has hampered studies on the genetics of resistance and plant breeding efforts to improve resistance. In the current study, rice hull medium, rice grains medium, and maize grain medium were used at 5%, 10% and 15% as a medium of inoculum for sheath blight infection under artificial screening. Significant difference was observed in the infection pattern of sheath blight between the different treatments. The appearance of the first lesion was observed in 6 days after sowing (DAS) in 15% rice hull medium and 16 days with rice grains medium (5%). Rice hull medium (15%) enabled the formation of sclerotia at 13 DAS and the disease incidence reached up to 90% at 21 DAS. The results suggests rice hull medium as an effective medium for rapid disease screening of rice genotypes for sheath blight resistance under greenhouse conditions.

Key words: Sheath blight, Rice, Inoculum, Disease Incidence, Sclerotia  

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Pralhad, S.P. and Krishnaraj, P.U., An Efficient Inoculation Method for Artificial Screening of Sheath Blight in Rice, Int. J. Pure App. Biosci.6(3): 44-48 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6589