Archives

International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 3
First page : (251) Last page : (264)
Article doi: : http://dx.doi.org/10.18782/2320-7051.6561

Effect of Various Factors (Temperature, pH, Nacl Conc., Carbon, Nitrogen and Amino Acids) on Growth of Pectinolytic Bacteria Isolated and Screened from Mango Fruit Yards

K. Sridevi¹, G.Venkatesh²*, M. Sumanth³, P. Sivaraagini⁴ and K. Vijayalakshmi^5
1Lecturer in Microbiology, SGS Arts College, Tirupati, A.P., India
*²Department of Microbiology, Dr Y.S.R. Horticultural University, Anantharajupeta, A.P., India
³M Sumanth B.Tech SASTRA University Thanjavur, India
⁴Lecturer in Microbiology, Smt NPS Govt College (w), Chittoor, A.P., India
⁵Retd Prof in Microbiology SPMVV, Tirupati, A.P., India
*Corresponding Author E-mail: parijathamamma@gmail.com
Received: 28.05.2018  |  Revised: 23.06.2018   |  Accepted: 28.xx.2018  

 

 ABSTRACT

Mango occupies a prominent place amongst the various fruits grown in India because of its great utility and health benefits and is acknowledged as the king of fruits. One of the agro-wastes currently causing pollution problems is the mango peel from fruit processing industries Mango peel is rich in carbohydrate, protein and pectin. Pectin is a major component peel of mango fruit. The degradation of mango peel by the bacterial populations is highly significant. The action of different extracellular enzymes on mango peel leads to the biodegradation of pectin. The type of enzymes is known as pectinolytic enzymes or pectinases which hydrolyses pectic substances readily. The isolates of B.megaterium were collected from different mango fruit processing industries around Tirupati, Chittoor district of Andhra Pradesh and it was isolated and screened on CPA medium under laboratory conditions. Effect of different pH levels, temperature, NaCl concentrations and effect of carbon, nitrogen and amino acids were tested against the growth of B.megaterium under in vitro. Results indicated that the growth of B. megaterium was maximum at pH range of 6.50-7.00 and temperature range of 35-40°C and NaCl concentration of 0.5 to 1%. And fructose and galactose as carbon sources, casein and peptone as nitrogen sources, phenyl alanine and histidine as the amino acids were observed as optimum for the growth of B. Megaterium respectively. Therefore, the main objective of the present study was to isolate, screen and to study the effect of various factors on pectinolytic activities of B. Megaterium from soils of decomposed fruit waste from mango processing industries.

Key words: Pectin, Mango peel, Pectinolytic enzymes or Pectinases, Temperature, pH, NaCl concentration, Carbon sources, Nitrogen sources, Amino acid sources.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sridevi, K., Venkatesh, G., Sumanth, M.,  Sivaraagini, P. and Vijayalakshmi, K., Effect of Various Factors (Temperature, pH, Nacl Conc., Carbon, Nitrogen and Amino Acids) on Growth of Pectinolytic Bacteria Isolated and Screened from Mango Fruit Yards, Int. J. Pure App. Biosci.6(3): 251-264 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6561