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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 2
First page : (1663) Last page : (1671)
Article doi: : http://dx.doi.org/10.18782/2320-7051.6367

Metabolite Profiling of In vitro Established Cultures of Picrorhiza
Kurroa Royle ex Benth in Different Growth Regime (s)

Durgesh Parihar, Alka Bameta, Anita Kumari, Geetanjali Kannojia and A. K. Gaur*
Laboratory of Bioprospecting and Metabolomics, Molecular Biology and Genetic Engineering,
Collage of Basic Sciences and Humanities, GBPUAT, Pantnagar-263145
*Corresponding Author E-mail: anilgaur123@rediffmail.com
Received: 14.03.2018  |  Revised: 21.04.2018   |  Accepted: 27.04.2018

 ABSTRACT

Picrorhiza Kurroa Royle ex Benth, a Scrophulariaceae member, is an endangered species according to Red Data Book and highly demanded medicinal herb due to established hepatoprotective function. Several monoterpenoid glycosides are present which are known as picroside I, picroside II, picroside III, more recently picroside IV and V are being identified specially for hepatomodulation in model laboratory animals and human.The plant has been over consumed for its valuable terpene glycosides. Biotechnological approaches through in vitro establishment of the important germplasm of Picrorhiza Kurroa Royle ex Benth at germplasm evaluation facility from suitable stage of its growth and development of leaf explants which in turn shall allow for the defined conditions for in vitro establishment under micro environment to look upon the production of picrosides in vitro. Present research work is an attempt toward quantification of Picroside(s) under regimes of various kind of phytoregulator(s) selected on the basis of growth behavior of callus. Callus cultures were established in vitro using leaves as an explant. TDZ (0.75 mg/L) + IBA (0.75 mg/L) was found better for callusing while BAP (0.50 mg/L) + Kinetin (0.75 mg/L) was found the best for shoot proliferation. Results of present study revealed that it might be possible that both mevalonate and non mevalonate pathways are involved in Picroside(s) biogenesis. GA3 seems to down regulate the biosynthesis of picrosides as GA3 also follows the same biosynthetic pathways as reported for picrosides. Electrophoresis of protein reveals bands like 84, 67, 58, 37 and 25 kDa which were observed in all samples taken at different days interval while other bands were seen in 12 and 18 days samples harvested from shoot tissue obtained from callus were subjected into suspension cultures, which implies that some proteins are produced under the increased concentration of GA3 in different suspension cultures at laboratory scale. Although the exact mechanism of picroside(s) metabolism requires to be explored in detail, however, our results are indicative of the increased amount of GA3 showed prominent effect on picroside(s) metabolic pathways.

Key words: In vitro propagation, Picrorhiza kurroa Royle ex Benth, Picroside (s).

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Parihar, D., Bameta, A., Kumari, A., Kannojia, G. and Gaur, A.K., Metabolite Profiling of in Vitro Established Cultures of Picrorhiza Kurroa Royle ex Benth in Different Growth Regime (s), Int. J. Pure App. Biosci.6(2): 1663-1671 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6367