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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2022, Volume : 10, Issue : 2
First page : (25) Last page : (28)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8888

Air Dry Technique for the Preparation of Meiotic Chromosomes from the Testes of Silkworm Bombyx mori L.

P. H. Thejaswini¹ and H. B. Mahesha^{2*   
1}Department of Biotechnology, Maharani’s Science College for Women, Mysuru-570 005
²Department of Sericulture, Yuvaraja’s College, University of Mysore, Mysore-570 005, India
*Corresponding Author E-mail: hbmseri@gmail.com
Received: 27.01.2022 | Revised: 14.03.2022 | Accepted: 22.03.2022 

 ABSTRACT

One strain of mulberry silkworm Bombyx mori L., namely NB4D2 at the age of the fifth instar, was selected as an experimental insect. During the fifth instar, testes were used to prepare meiotic chromosomes. The air dry technique for preparing meiotic chromosomes was developed and standardised. Both temporary and permanent micro slides showed clear different stages of meiosis. The different meiotic stages were observed under 1000x magnification and photographed using conventional photographic techniques.

Keywords: Silkworm, Testes, Gonial Metaphase, Meiotic Chromosomes.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Thejaswini, P. H., & Mahesha, H. B. (2022). Air Dry Technique for the Preparation of Meiotic Chromosomes from the Testes of Silkworm Bombyx mori L., Ind. J. Pure App. Biosci.10(2), 25-28. doi: http://dx.doi.org/10.18782/2582-2845.8888