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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2022, Volume : 10, Issue : 2
First page : (1) Last page : (9)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8886

Effect of Environmental Temperature on Cypovirus Multiplication and Biomolecules in Silkworm Bombyx mori L.

P. H. Thejaswini¹ and H. B. Mahesha^{2*   
1}Department of Biotechnology, Maharani’s Science College for Women, Mysuru-570 005
²Department of Sericulture, Yuvaraja’s College, University of Mysore, Mysore-570 005, India
*Corresponding Author E-mail: hbmseri@gmail.com
Received: 18.01.2022 | Revised: 10.03.2022 | Accepted: 18.03.2022 

 ABSTRACT

Two strains of mulberry silkworm, namely Pure Mysore and NB4D2, at fifth instar first day were inoculated with 40ml of BmCPV suspension from the stock of 1.215 X 106 PIB/ml in 0.75% of NaCl solution by ‘oral injection’. Three batches of such inoculated worms were allowed to continue larval development at 20±1°C, 24±1°C and 30±1°C in separate BOD incubators. The crude extract of midgut tissue was prepared from the survivals and used to count the total number of polyhedra per larva in each batch. Further, during virus multiplication at different rearing temperatures, the total amount of glucose, proteins and amylase as well as succinate dehydrogenase activity levels in haemolymph were studied. The experimental results clearly indicated that the atmospheric temperature has a direct influence on a number of polyhedral formations and biomolecules.

Keywords: Silkworm, Cypovirus, Protein, Glucose, Amylase, Succinate dehydrogenase.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Thejaswini, P. H., & Mahesha, H. B. (2022). Effect of Environmental Temperature on Cypovirus Multiplication and Biomolecules in Silkworm Bombyx mori l., Ind. J. Pure App. Biosci.10(2), 1-9. doi: http://dx.doi.org/10.18782/2582-2845.8886