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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2022, Volume : 10, Issue : 1
First page : (29) Last page : (39)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8861

Saccharification of Used Paper Cup for Ethanol Production by Yeast Isolate

Navneet Kumari¹, Komal¹, Surender Singh² and Kirpa Ram^1*
1Department of Botany, Baba Mastnath University, Rohtak- 124021
²Pt. NRS Govt. College, MDU, Rohtak- 124001
*Corresponding Author E-mail: dr.kirparamjangra@gmail.com
Received: 15.12.2021 | Revised: 17.02.2022 | Accepted: 25.02.2022 

 ABSTRACT

The main aim of this work was to develop an effective method for pretreatment and enzymatic saccharification of cellulose and hemicellulose components into fermentable sugars, as well as fermentation of the hydrolysate to bioethanol by new yeast isolates. YAJ-2M (3.2%), YCJ-1 (3.4%) and YAJ-2M 11 (5.6%) produced maximum ethanol. UV- induction responsible for genetic improvement of yeast isolates for ethanol production.  Pretreatment of paper with alkali- acid reduced the lignin content from 25% to 14.3%. Maximum liberation of reducing sugars (168.7 μg/ml) was attained at 50°C for 96 hours at 150 rpm using enzyme dose of 20 U/g in alkali-acid (sodium hydroxide-orthophosphoric acid) pretreated paper cup. Fermentation of hydrolysate paper by yeast isolates (YAJ-1 M2 11 mutant) at 30°C, pH 4.7, 150 rpm and resulted in 1% ethanol after 96 h. Present investigation was found mutant YAJ-1 M2 11 produced highest ethanol concentration in hydrolysed paper than other tested mutant.

Key words: Yeast Isolates, Paper Cup, Ethanol, Saccharification, Fermentation.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumari, N., Komal, Singh, S., & Ram, K. (2022). Saccharification of Used Paper Cup for Ethanol Production by Yeast Isolate, Ind. J. Pure App. Biosci.10(1), 29-39. doi: http://dx.doi.org/10.18782/2582-2845.8861